NAD+ 500 MG

NAD+ 500 mg by Peptide Hubs: The Essential Coenzyme for Cellular Energy & Longevity Research

Peptide Hubs presents its premium research-grade NAD+ 500 mg, offering the highest concentration of Nicotinamide Adenine Dinucleotide available for advanced investigative studies into cellular metabolism, mitochondrial function, and the biology of aging. NAD+ is not a peptide but a fundamental coenzyme present in every cell, crucial for over 500 enzymatic reactions, including those central to energy production (ATP synthesis), DNA repair, and cellular defense. With age and metabolic stress, NAD+ levels decline precipitously, a phenomenon linked to reduced mitochondrial efficiency, cellular senescence, and increased vulnerability to disease. Our high-dose 500 mg lyophilized powder provides researchers with a potent tool to explore the effects of NAD+ repletion on energy metabolism, exercise performance, cognitive function, and systemic rejuvenation. Sourced and manufactured under rigorous controls in a USA facility, this product represents the forefront of accessible, high-purity metabolic research compounds.

Mechanism of Action and Research Effects

NAD+ functions as a vital electron carrier in redox reactions, shuttling electrons during the conversion of nutrients into cellular energy (ATP) within the mitochondria. Its role extends far beyond energy production. NAD+ is the required substrate for critical longevity-associated enzymes called sirtuins (SIRT1-7), which regulate gene expression, DNA repair, and stress resistance. It also fuels PARP enzymes involved in DNA repair and CD38 for calcium signaling. In research settings, elevating NAD+ levels has been associated with profound effects:

• Enhanced Mitochondrial Biogenesis & Function: Increased NAD+ activates SIRT1 and PGC-1α, signaling the creation of new, healthy mitochondria and improving the efficiency of existing ones, leading to greater cellular energy output.
• Improved Metabolic Flexibility: Supports the body's ability to switch between fuel sources (carbohydrates and fats), a key factor in endurance and body composition.
• Activation of Cellular Repair Pathways: Fuels DNA repair mechanisms via PARP and promotes autophagy (cellular "clean-up") through sirtuin activation.
• Neuroprotective & Cognitive Benefits: Supports neuronal health and function, with research models showing potential for improved memory and protection against age-related cognitive decline.
• Cardiovascular & Metabolic Health: Supports endothelial function and healthy aging of the vascular system. The foundational science of NAD+ is well-documented in resources like the NIH PubMed database.

Research Dosage and Administration Protocol

NAD+ 500 mg is typically administered via intravenous (IV) drip, intramuscular (IM), or subcutaneous (SubQ) injection in research settings, with the route significantly impacting bioavailability and effect profile. IV administration provides the most immediate and complete systemic saturation but requires specialized equipment. For laboratory studies, intramuscular or subcutaneous protocols are more common. Research dosages vary widely based on the model and objective. Common investigative ranges are between 50 mg to 500 mg per administration. For high-dose protocols, the 500 mg vial allows for efficient dosing. Reconstitution is performed with sterile bacteriostatic water or saline. For example, adding 2.5 mL to the 500 mg vial yields a concentration of 200 mg/mL. A 0.5 mL (100 mg) injection would then be drawn from this solution. Frequency can range from daily to 2-3 times per week, depending on the research design aimed at maintaining elevated NAD+ levels. Strict aseptic technique is non-negotiable.

Recommended Research Cycle and Synergistic Stacks

Given its role in fundamental cellular processes, NAD+ research cycles can be highly variable. For studying acute effects on energy and recovery, short cycles of 2-4 weeks with frequent dosing may be used. For investigating longer-term impacts on gene expression and anti-aging biomarkers, cycles of 8-12 weeks or longer are common. NAD+ synergizes powerfully with other compounds that support mitochondrial health and longevity. Researchers often combine it with Epitalon 50 mg to study synergistic effects on telomere maintenance and pineal gland regulation. For comprehensive cellular protection and antioxidant support, stacking with L-Glutathione 600 mg is logical. To explore enhanced metabolic and endurance adaptations, combination with MOTS-C 10 mg, a mitochondrial-derived peptide, is a cutting-edge area of study. For research focused on tissue repair and inflammation modulation post-exercise, adding BPC 157 can create a robust recovery protocol. Furthermore, to support the structural health of joints and connective tissues under metabolic stress, Chonluten 20 mg can be incorporated into the research design.

Possible Side Effects and Research Considerations

In research models, NAD+ is generally well-tolerated as it is an endogenous molecule. However, rapid administration, particularly via IV, can cause transient side effects related to vasodilation and the flushing of metabolites. These may include a warm flushing sensation in the face and chest, mild nausea, lightheadedness, or fatigue, which typically pass within 30-60 minutes. Slower infusion rates or switching to IM/SubQ routes often mitigate these effects. There is no evidence of androgenic, estrogenic, or progestogenic activity—no acne, water retention, or HBP from hormonal mechanisms. On the contrary, it may support cardiovascular health. It is not hepatotoxic; in fact, it may support liver function. There is no aromatization or HPTA suppression. Researchers should monitor for signs of localized irritation at injection sites. The primary consideration is that high doses may temporarily alter redox state, so comprehensive metabolic monitoring is advised. This compound is strictly for controlled laboratory research.

NAD+ Decline and the "Hallmarks of Aging"

The interest in NAD+ supplementation in research is directly tied to its central role in countering several recognized "hallmarks of aging." The age-related decline in NAD+ contributes to:

• Mitochondrial Dysfunction: Reduced energy output and increased oxidative stress.
• Genomic Instability: Impaired DNA repair capability.
• Epigenetic Alterations: Dysregulated gene expression via sirtuin inactivity.
• Loss of Proteostasis: Reduced clearance of damaged proteins.
• Cellular Senescence: Accumulation of non-dividing, inflammatory cells.

By repleting NAD+, researchers are investigating interventions that may simultaneously address multiple of these pathways, making it a cornerstone molecule in the field of geroscience and performance longevity.

Quality, Purity, and Research Viability

The stability and potency of NAD+ are paramount for credible research. Peptide Hubs' NAD+ 500 mg is synthesized to the highest pharmaceutical standards, lyophilized to ensure long-term stability, and sealed under inert gas to prevent oxidation. Each batch is subjected to rigorous analytical testing via HPLC to confirm purity (>99%) and the absence of degradation products like nicotinamide. Mass spectrometry verifies molecular identity. This commitment to quality ensures that researchers are working with a fully active, reliable coenzyme, allowing for the generation of reproducible and meaningful data in the study of cellular energetics and longevity science.

Frequently Asked Questions